Bidirectionalization of a methyl jasmonate-inducible plant promoter.
Identifieur interne : 002F88 ( Main/Exploration ); précédent : 002F87; suivant : 002F89Bidirectionalization of a methyl jasmonate-inducible plant promoter.
Auteurs : Huiquan Zheng [République populaire de Chine] ; Yang Lei ; Shanzhi Lin ; Qian Zhang ; Zhiyi ZhangSource :
- Biotechnology letters [ 1573-6776 ] ; 2011.
Descripteurs français
- KwdFr :
- Activation de la transcription (MeSH), Acétates (métabolisme), Cyclopentanes (métabolisme), Fusion artificielle de gènes (MeSH), Glucuronidase (génétique), Glucuronidase (métabolisme), Gènes rapporteurs (MeSH), Oxylipines (métabolisme), Populus (génétique), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Recombinaison génétique (MeSH), Rhizobium (génétique), Régions promotrices (génétique) (effets des médicaments et des substances chimiques).
- MESH :
- effets des médicaments et des substances chimiques : Régions promotrices (génétique).
- génétique : Glucuronidase, Populus, Protéines à fluorescence verte, Rhizobium.
- métabolisme : Acétates, Cyclopentanes, Glucuronidase, Oxylipines, Protéines à fluorescence verte.
- Activation de la transcription, Fusion artificielle de gènes, Gènes rapporteurs, Recombinaison génétique.
English descriptors
- KwdEn :
- Acetates (metabolism), Artificial Gene Fusion (MeSH), Cyclopentanes (metabolism), Genes, Reporter (MeSH), Glucuronidase (genetics), Glucuronidase (metabolism), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Oxylipins (metabolism), Populus (genetics), Promoter Regions, Genetic (drug effects), Recombination, Genetic (MeSH), Rhizobium (genetics), Transcriptional Activation (MeSH).
- MESH :
- chemical , genetics : Glucuronidase, Green Fluorescent Proteins.
- chemical , metabolism : Acetates, Cyclopentanes, Glucuronidase, Green Fluorescent Proteins, Oxylipins.
- drug effects : Promoter Regions, Genetic.
- genetics : Populus, Rhizobium.
- Artificial Gene Fusion, Genes, Reporter, Recombination, Genetic, Transcriptional Activation.
Abstract
A unidirectional promoter can be transformed into a bidirectional module by artificial methods. Here we report the bidirectionalization of the methyl jasmonate (MeJA)-inducible PtDrl02 promoter derived from poplar [(Populus tomentosa × P. bolleana) × P. tomentosa] in planta. Construction of the bidirectional PtDrl02 promoter (designated as mPtDrl02) was rapidly achieved by introducing a minimal 35S promoter in the opposite orientation to the 5' end of PtDrl02. β-Glucuronidase (GUS) and green fluorescent protein (GFP) reporter genes were also interchangeably linked to the 3'-and 5'-ends of mPtDrl02 to produce GFP/mPtDrl02/GUS and GUS/mPtDrl02/GFP vectors, respectively. Using the Agrobacterium-mediated transient expression approach, we demonstrated that the mPtDrl02 module was able to drive gene (GUS and GFP) expression in both orientations simultaneously. Furthermore, the cooperative and concurrent activity from both directions of the mPtDrl02 module was demonstrated following MeJA induction. To our knowledge, this is the first report of an artificial MeJA-responsive bidirectional promoter in perennial plants.
DOI: 10.1007/s10529-010-0431-5
PubMed: 20953667
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Bidirectionalization of a methyl jasmonate-inducible plant promoter.</title><author><name sortKey="Zheng, Huiquan" sort="Zheng, Huiquan" uniqKey="Zheng H" first="Huiquan" last="Zheng">Huiquan Zheng</name><affiliation wicri:level="1"><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lei, Yang" sort="Lei, Yang" uniqKey="Lei Y" first="Yang" last="Lei">Yang Lei</name></author><author><name sortKey="Lin, Shanzhi" sort="Lin, Shanzhi" uniqKey="Lin S" first="Shanzhi" last="Lin">Shanzhi Lin</name></author><author><name sortKey="Zhang, Qian" sort="Zhang, Qian" uniqKey="Zhang Q" first="Qian" last="Zhang">Qian Zhang</name></author><author><name sortKey="Zhang, Zhiyi" sort="Zhang, Zhiyi" uniqKey="Zhang Z" first="Zhiyi" last="Zhang">Zhiyi Zhang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:20953667</idno><idno type="pmid">20953667</idno><idno type="doi">10.1007/s10529-010-0431-5</idno><idno type="wicri:Area/Main/Corpus">003037</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003037</idno><idno type="wicri:Area/Main/Curation">003037</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003037</idno><idno type="wicri:Area/Main/Exploration">003037</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Bidirectionalization of a methyl jasmonate-inducible plant promoter.</title><author><name sortKey="Zheng, Huiquan" sort="Zheng, Huiquan" uniqKey="Zheng H" first="Huiquan" last="Zheng">Huiquan Zheng</name><affiliation wicri:level="1"><nlm:affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lei, Yang" sort="Lei, Yang" uniqKey="Lei Y" first="Yang" last="Lei">Yang Lei</name></author><author><name sortKey="Lin, Shanzhi" sort="Lin, Shanzhi" uniqKey="Lin S" first="Shanzhi" last="Lin">Shanzhi Lin</name></author><author><name sortKey="Zhang, Qian" sort="Zhang, Qian" uniqKey="Zhang Q" first="Qian" last="Zhang">Qian Zhang</name></author><author><name sortKey="Zhang, Zhiyi" sort="Zhang, Zhiyi" uniqKey="Zhang Z" first="Zhiyi" last="Zhang">Zhiyi Zhang</name></author></analytic><series><title level="j">Biotechnology letters</title><idno type="eISSN">1573-6776</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetates (metabolism)</term><term>Artificial Gene Fusion (MeSH)</term><term>Cyclopentanes (metabolism)</term><term>Genes, Reporter (MeSH)</term><term>Glucuronidase (genetics)</term><term>Glucuronidase (metabolism)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Oxylipins (metabolism)</term><term>Populus (genetics)</term><term>Promoter Regions, Genetic (drug effects)</term><term>Recombination, Genetic (MeSH)</term><term>Rhizobium (genetics)</term><term>Transcriptional Activation (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation de la transcription (MeSH)</term><term>Acétates (métabolisme)</term><term>Cyclopentanes (métabolisme)</term><term>Fusion artificielle de gènes (MeSH)</term><term>Glucuronidase (génétique)</term><term>Glucuronidase (métabolisme)</term><term>Gènes rapporteurs (MeSH)</term><term>Oxylipines (métabolisme)</term><term>Populus (génétique)</term><term>Protéines à fluorescence verte (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Recombinaison génétique (MeSH)</term><term>Rhizobium (génétique)</term><term>Régions promotrices (génétique) (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucuronidase</term><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acetates</term><term>Cyclopentanes</term><term>Glucuronidase</term><term>Green Fluorescent Proteins</term><term>Oxylipins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Régions promotrices (génétique)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glucuronidase</term><term>Populus</term><term>Protéines à fluorescence verte</term><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acétates</term><term>Cyclopentanes</term><term>Glucuronidase</term><term>Oxylipines</term><term>Protéines à fluorescence verte</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Artificial Gene Fusion</term><term>Genes, Reporter</term><term>Recombination, Genetic</term><term>Transcriptional Activation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation de la transcription</term><term>Fusion artificielle de gènes</term><term>Gènes rapporteurs</term><term>Recombinaison génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A unidirectional promoter can be transformed into a bidirectional module by artificial methods. Here we report the bidirectionalization of the methyl jasmonate (MeJA)-inducible PtDrl02 promoter derived from poplar [(Populus tomentosa × P. bolleana) × P. tomentosa] in planta. Construction of the bidirectional PtDrl02 promoter (designated as mPtDrl02) was rapidly achieved by introducing a minimal 35S promoter in the opposite orientation to the 5' end of PtDrl02. β-Glucuronidase (GUS) and green fluorescent protein (GFP) reporter genes were also interchangeably linked to the 3'-and 5'-ends of mPtDrl02 to produce GFP/mPtDrl02/GUS and GUS/mPtDrl02/GFP vectors, respectively. Using the Agrobacterium-mediated transient expression approach, we demonstrated that the mPtDrl02 module was able to drive gene (GUS and GFP) expression in both orientations simultaneously. Furthermore, the cooperative and concurrent activity from both directions of the mPtDrl02 module was demonstrated following MeJA induction. To our knowledge, this is the first report of an artificial MeJA-responsive bidirectional promoter in perennial plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20953667</PMID><DateCompleted><Year>2011</Year><Month>04</Month><Day>14</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-6776</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>2</Issue><PubDate><Year>2011</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Biotechnology letters</Title><ISOAbbreviation>Biotechnol Lett</ISOAbbreviation></Journal><ArticleTitle>Bidirectionalization of a methyl jasmonate-inducible plant promoter.</ArticleTitle><Pagination><MedlinePgn>387-93</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10529-010-0431-5</ELocationID><Abstract><AbstractText>A unidirectional promoter can be transformed into a bidirectional module by artificial methods. Here we report the bidirectionalization of the methyl jasmonate (MeJA)-inducible PtDrl02 promoter derived from poplar [(Populus tomentosa × P. bolleana) × P. tomentosa] in planta. Construction of the bidirectional PtDrl02 promoter (designated as mPtDrl02) was rapidly achieved by introducing a minimal 35S promoter in the opposite orientation to the 5' end of PtDrl02. β-Glucuronidase (GUS) and green fluorescent protein (GFP) reporter genes were also interchangeably linked to the 3'-and 5'-ends of mPtDrl02 to produce GFP/mPtDrl02/GUS and GUS/mPtDrl02/GFP vectors, respectively. Using the Agrobacterium-mediated transient expression approach, we demonstrated that the mPtDrl02 module was able to drive gene (GUS and GFP) expression in both orientations simultaneously. Furthermore, the cooperative and concurrent activity from both directions of the mPtDrl02 module was demonstrated following MeJA induction. To our knowledge, this is the first report of an artificial MeJA-responsive bidirectional promoter in perennial plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Huiquan</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lei</LastName><ForeName>Yang</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Shanzhi</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Qian</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Zhiyi</ForeName><Initials>Z</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>10</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biotechnol Lett</MedlineTA><NlmUniqueID>8008051</NlmUniqueID><ISSNLinking>0141-5492</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000085">Acetates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003517">Cyclopentanes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054883">Oxylipins</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>900N171A0F</RegistryNumber><NameOfSubstance UI="C072239">methyl jasmonate</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000085" MajorTopicYN="N">Acetates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019677" MajorTopicYN="N">Artificial Gene Fusion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003517" MajorTopicYN="N">Cyclopentanes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054883" MajorTopicYN="N">Oxylipins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011995" MajorTopicYN="N">Recombination, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015533" MajorTopicYN="Y">Transcriptional Activation</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>09</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>09</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>10</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>10</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20953667</ArticleId><ArticleId IdType="doi">10.1007/s10529-010-0431-5</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Lei, Yang" sort="Lei, Yang" uniqKey="Lei Y" first="Yang" last="Lei">Yang Lei</name><name sortKey="Lin, Shanzhi" sort="Lin, Shanzhi" uniqKey="Lin S" first="Shanzhi" last="Lin">Shanzhi Lin</name><name sortKey="Zhang, Qian" sort="Zhang, Qian" uniqKey="Zhang Q" first="Qian" last="Zhang">Qian Zhang</name><name sortKey="Zhang, Zhiyi" sort="Zhang, Zhiyi" uniqKey="Zhang Z" first="Zhiyi" last="Zhang">Zhiyi Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zheng, Huiquan" sort="Zheng, Huiquan" uniqKey="Zheng H" first="Huiquan" last="Zheng">Huiquan Zheng</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002F88 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002F88 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20953667
|texte= Bidirectionalization of a methyl jasmonate-inducible plant promoter.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20953667" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |